Human Umbilical Mesenchymal Stem Cells-Derived Microvesicles Attenuate Formation of Hypertrophic Scar through Multiple Mechanisms

Mesenchymal stem cells and the derived extracellular microvesicles are potential promising therapy for many disease conditions, including wound healing. Since current therapeutic approaches do not satisfactorily attenuate or ameliorate formation of hypertrophic scars, it is necessary to develop novel drugs achieve better outcomes. In this study, we investigated effects underlying mechanisms human umbilical mesenchymal (HUMSCs)-derived (HUMSCs-MVs) on scar using a rabbit ear model foreskin fibroblasts (HFF) culture model. The results showed that HUMSCs-MVs reduced tissues in based appearance observation, hematoxylin eosin (H&E), Masson, immunohistochemical stainings. inhibited invasion HFF decreased levels α-SMA, N-WASP, cortacin proteins. also cell proliferation cells. MMP-1, MMP-3, TIMP-3 mRNA were significantly increased, TIMP-4 level NF-kB p65/β-catenin protein after treatment. p-SMAD2/3 ratios p-SMAD2/3/SMAD2/3 both healing CD34 VEGF-A was magnitudes changes these markers by mostly higher than those dexamethasone. These suggested attenuated during through inhibiting fibrotic cells, inflammation oxidative stress, Smad2/3 activation, angiogenesis. drug